Characterization of isoform expression and subcellular distribution of MYPT1 in intestinal epithelial cells

• Full length and variant 2 of MYPT1 are expressed in human intestinal epithelial cells.
• Full length and variant 2 of MYPT1 are expressed in isolated mouse IECs.
• MYPT1 is distributed at cell–cell contacts in confluent Caco-2 cells.

The regulation of intestinal epithelial permeability requires phosphorylation of myosin regulatory light chain (MLC). The phosphorylation status of MLC is regulated by myosin light chain phosphatase (MLCP) activities. The activity of the catalytic subunit of MLCP (PP1cδ) toward MLC depends on its regulatory subunit (MYPT1). In this study, we revealed the presence of two MYPT1 isoforms, full length and variant 2 in human intestinal (Caco-2) epithelial cells and isolated intestinal epithelial cells (IECs) from mice. In confluent Caco-2 cells, MYPT1 was distributed at cell–cell contacts and colocalized with F-actin. These results suggest that MYPT1 isoforms are expressed in intestinal epithelial cells and MYPT1 may be involved in the regulation of intestinal epithelial barrier function.