کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2815352 1159866 2015 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of hub glycogenes and their nsSNP analysis from mouse RNA-Seq data
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Identification of hub glycogenes and their nsSNP analysis from mouse RNA-Seq data
چکیده انگلیسی


• We create an interaction network of 923 glycogenes detected from mouse RNA-Seq data.
• These glycogenes play roles in immune response and various metabolic processes.
• We identify hub glycogenes from the network.
• Coding nsSNPs were identified for hubs by computational analysis.

Glycogenes regulate a large number of biological processes such as cancer and development. In this work, we created an interaction network of 923 glycogenes to detect potential hubs from different mouse tissues using RNA-Seq data. DAVID functional cluster analysis revealed enrichment of immune response, glycoprotein and cholesterol metabolic processes. We also explored nsSNPs that may modify the expression and function of identified hubs using computational methods. We observe that the number of nsSNPs predicted by any two methods to affect protein function is 4, 7 and 2 for FLT1, NID2 and TNFRSF1B. Residues in the native and mutant proteins were analyzed for solvent accessibility and secondary structure change. Analysis of hubs can help in determining their degree of conservation and understanding their functions in biological processes. The nsSNPs proposed in this work may be further targeted through experimental methods for understanding structural and functional relationships of hub mutants.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 574, Issue 2, 15 December 2015, Pages 235–246
نویسندگان
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