کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2815406 | 1159868 | 2016 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Roles of miR-278-3p in IBP2 regulation and Bombyx mori cytoplasmic polyhedrosis virus replication Roles of miR-278-3p in IBP2 regulation and Bombyx mori cytoplasmic polyhedrosis virus replication](/preview/png/2815406.png)
• The first report on function of bmo-miRNAs in response to BmCPV infection
• IBP2 is a target gene of miR-278-3p in silkworm.
• Overexpression of miR-278-3p negatively regulates IBP2 expression in silkworm larvae.
• Overexpression of miR-278-3p facilitates BmCPV replication.
Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) is a major viral pathogen of silkworm and remains a big challenge to the sericultural industry. Insulin-related peptide binding protein 2 (IBP2) gene, induced by BmCPV infection may play an important role in B. mori immune response. MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression at the post-transcriptional level and play an important role in various processes, including immunity and antiviral response. In this study, we identified IBP2 as one of the targets for miR-278-3p by using luciferase reporter assay. Overexpression of miR-278-3p negatively regulates the expression of IBP2 in silkworm larvae and positively regulates the mRNA transcript level of BmCPV. Our results suggest that miR-278-3p may play an important role in BmCPV replication. It's the first report on bmo-miRNAs in response to BmCPV infection and could provide a new clue to explore the molecular mechanism of BmCPV infection and host immunity.
Journal: Gene - Volume 575, Issue 2, Part 1, 10 January 2016, Pages 264–269