کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2815494 1159873 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of novel alternative splicing transcript and expression analysis of bovine TMEM95 gene
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Identification of novel alternative splicing transcript and expression analysis of bovine TMEM95 gene
چکیده انگلیسی


• Two transcripts of TMEM95 were identified in male fetus cattle.
• TMEM95-SV1 maybe closely linked with proteins interaction and acrosome reaction.
• The two transcripts exclusively expressed in the testes and brain.

Transmembrane protein 95 (TMEM95) is closely related to male reproductive performance in cattle, but does not affect semen quality. Alternative splicing plays an important role in regulating biological function as well as in generating proteomic and functional diversity in metazoan organisms. Thus, the aim of this study was to clone and identify transcripts of the TMEM95 gene in cattle using RT-PCR, characterize them via bioinformatics analysis, and detect their expression patterns using qRT-PCR. Two transcripts of TMEM95 were identified in cattle, including TMEM95-SV1 and TMEM95-SV2. Bioinformatics predicted that TMEM95-SV1 has a leucine-rich repeat C-terminal domain and a Pfam: IZUMO. These regions are closely related to protein interactions and the acrosome reaction, respectively. Interestingly, the two transcripts were exclusively expressed in the testes and brain in male fetus cattle, and TMEM95-SV1 was expressed in the brain at significantly higher levels than in the testis (P < 0.05, 4.06-fold) and TMEM95-SV2 in the brain (P < 0.05, 4.95-fold). These findings enrich the understanding of the TMEM95 gene function and benefit for enhancing male reproduction in cattle industry.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 575, Issue 2, Part 2, 10 January 2016, Pages 531–536
نویسندگان
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