کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2815691 1159887 2015 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification and characterization of reproductive KRAB-ZF genes in mice
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Identification and characterization of reproductive KRAB-ZF genes in mice
چکیده انگلیسی


• We systematically identified KRAB-ZF genes expressed in the mouse gonads.
• Zfp819 is expressed specifically in spermatogenic cells.
• Overexpression of Zfp819 affected cell proliferation and induced apoptosis.
• Microarray analysis identified numerous Zfp819-downstream genes having potential functions in spermatogenesis and apoptosis.

The mammalian genome contains numerous genes encoding transcription factors that contain Krüppel-associated box (KRAB) and C2H2-type zinc finger (ZF) motifs (KRAB-ZF). In the present study, we identified KRAB-ZF genes expressed in the mouse testis or ovary, and selected three genes that exhibit gonad-specific or gonad-predominant expression. In vitro analyses showed that these gonadal KRAB-ZF proteins are localized in cell nuclei and are able to repress transcriptional activity. We further analyzed one of the gonad-specific reproductive genes, Zfp819, and found that it is expressed exclusively in spermatogenic cells. Overexpression of Zfp819 suppressed cell proliferation and induced apoptosis. Microarray analysis of Zfp819-overexpressing cells allowed us to identify numerous, potential target genes. A number of the down-regulated genes were found to show gene expression levels inversely correlated with Zfp819 during spermatogenesis. Some of the down-regulated genes were previously reported to play significant roles in spermatogenesis and apoptosis. Collectively, our study provides the first comprehensive information regarding the expression of reproductive KRAB-ZF genes in mice and reveals potential functions of Zfp819.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 565, Issue 1, 1 July 2015, Pages 45–55
نویسندگان
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