کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2816069 | 1159913 | 2014 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Isolation and in silico analysis of Fe-superoxide dismutase in the cyanobacterium Nostoc commune Isolation and in silico analysis of Fe-superoxide dismutase in the cyanobacterium Nostoc commune](/preview/png/2816069.png)
• Nostoc commune has SOD and catalase as arsenal against oxidative stress.
• High conservation of N and C-terminal domain regions in cyanobacterial Fe-SOD
• H28, H80, D162 and H166 Fe-metal binding sites are conserved in Fe-SOD.
• Homology model of Fe-SOD in Nostoc commune was validated as good quality model.
• Fe-SOD of N. punctiforme and N. commune has close evolutionary homology.
Cyanobacteria are known to endure various stress conditions due to the inbuilt potential for oxidative stress alleviation owing to the presence of an array of antioxidants. The present study shows that Antarctic cyanobacterium Nostoc commune possesses two antioxidative enzymes viz., superoxide dismutase (SOD) and catalase that jointly cope with environmental stresses prevailing at its natural habitat. Native-PAGE analysis illustrates the presence of a single prominent isoform recognized as Fe-SOD and three distinct isoforms of catalase. The protein sequence of Fe-SOD in N. commune retrieved from NCBI protein sequence database was used for in silico analysis. 3D structure of N. commune was predicted by comparative modeling using MODELLER 9v11. Further, this model was validated for its quality by Ramachandran plot, ERRAT, Verify 3D and ProSA-web which revealed good structure quality of the model. Multiple sequence alignment showed high conservation in N and C-terminal domain regions along with all metal binding positions in Fe-SOD which were also found to be highly conserved in all 28 cyanobacterial species under study, including N. commune. In silico prediction of isoelectric point and molecular weight of Fe-SOD was found to be 5.48 and 22,342.98 Da respectively. The phylogenetic tree revealed that among 28 cyanobacterial species, Fe-SOD in N. commune was the closest evolutionary homolog of Fe-SOD in Nostoc punctiforme as evident by strong bootstrap value. Thus, N. commune may serve as a good biological model for studies related to survival of life under extreme conditions prevailing at the Antarctic region. Moreover cyanobacteria may be exploited for biochemical and biotechnological applications of enzymatic antioxidants.
Journal: Gene - Volume 553, Issue 2, 15 December 2014, Pages 117–125