کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2816355 | 1159928 | 2014 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Methylation-associated silencing of microRNA-34b in hepatocellular carcinoma cancer Methylation-associated silencing of microRNA-34b in hepatocellular carcinoma cancer](/preview/png/2816355.png)
• A high frequency of miR-34b/c methylation was observed in HCC tissues.
• miR-34a and miR-34b were significantly down-regulated in HCC tissues.
• The expression of miR-34b was associated with the methylation in HCC tissues.
MicroRNAs (miRNAs) can act as oncogenes or tumor-suppressor genes in human cancers including HCC. Previous studies have identified miR-34 family as an important component of the tumor suppressor network during carcinogenesis. In this study, we investigated the methylation status of miR-34 family in HCC tumor and adjacent non-tumor tissues using methylation-specific PCR (MSP). The methylation frequencies of miR-34a and miR-34b/c were 72.1% (31/43) and 79.1% (34/43) in HCC tissues, which were significantly higher than that in the adjacent non-tumor tissues (P < 0.05), respectively. The results were validated by bisulfite sequencing PCR (BSP). Quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis showed that the expression of miR-34a and miR-34b was significantly down-regulated in HCC tissues compared with adjacent non-tumor tissues (P < 0.05). Moreover, the expression of miR-34b was inversely correlated to CpG island methylation in tumor tissues, but not for miR-34a. In summary, our results suggest that DNA methylation may be involved in the inactivation of miR-34b in HCC.
Journal: Gene - Volume 543, Issue 1, 10 June 2014, Pages 101–107