کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2816562 1159941 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular characterization, transcriptional regulation and association analysis with carcass traits of porcine TCAP gene
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Molecular characterization, transcriptional regulation and association analysis with carcass traits of porcine TCAP gene
چکیده انگلیسی


• We have isolated and characterized the porcine TCAP gene.
• We detected the expression level of pig TCAP gene by RT-PCR.
• We found a SNP and did association analysis with carcass traits.
• Proximal promoter of TCAP was isolated and analyzed for transcriptional activity.
• Putative transcription factor (MyoD, MyoG and MEF2) enhance transcription

TCAP (also known as titin-cap or telethonin) is one of the titin interacting Z-disk proteins involved in the regulation and development of normal sarcomeric structure. In this study, we cloned the cDNA and promoter sequences of porcine TCAP gene, which contained a 504 bp full-length coding region. Quantitative real-time PCR (qRT-PCR) analyses showed that porcine TCAP was highly expressed in the skeletal muscle, heart, and kidney. During postnatal muscle development, TCAP expression was down-regulated from 30 days to 120 days in Large White and Meishan pigs. One single nucleotide polymorphism c.334G>A in exon 2 of the TCAP gene was identified and detected by allele-specific primer-polymerase chain reaction (ASP-PCR). Association analysis revealed that the polymorphism had significant associations (P < 0.05 and P < 0.01) with some carcass traits. Analysis of the porcine TCAP promoter in different cell lines demonstrated that it is a muscle-specific promoter. In addition, we found that the porcine TCAP promoter can be activated by MyoD, MyoG and MEF2 in myotubes, which indicated that TCAP may play a role in the regulation of porcine skeletal muscle development. These findings provide useful information for the further investigation of the function of TCAP in porcine skeletal muscle.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 538, Issue 2, 1 April 2014, Pages 273–279
نویسندگان
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