کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2816719 | 1159950 | 2014 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Selected isomiR expression profiles via arm switching? Selected isomiR expression profiles via arm switching?](/preview/png/2816719.png)
• Less miR-#-5p/3p are simultaneously identified as abundant miRNA species.
• The two arms of pre-miRNAs show various isomiR expression profiles.
• The diversity of isomiR profiles may be influenced by the “arm switching”.
A mature miRNA may be generated from 5p or 3p arm of a hairpin precursor. The selection may be flexible via “arm switching”. However, accumulating evidences suggest that both arms of many pre-miRNAs can yield mature functional miRNAs. Herein, we attempted to compare the isomiR expression profiles between the two arms through analyzing in-house and published small RNA deep sequencing datasets. Although many miR-#-5p and miR-#-3p have been reported as functional miRNAs, fewer miRNA pairs (11 and 6 pairs are collected in tumor and normal cells, respectively) are simultaneously identified as abundant miRNA species. According to isomiR types and dominant isomiR species, miR-#-5p and miR-#-3p show various isomiR expression profiles as well as diverse enrichment levels. IsomiR profiles of non-dominant arm are not well-conserved in 5′ ends as well as isomiR profiles of dominant arm. If both the miR-#-5p and miR-#-3p are abundantly expressed, their isomiR expression profiles are always stable across different samples. Similar to diverse enrichment levels of miR-#-5p and miR-#-3p, the isomiR expression patterns may also be influenced by the phenomenon of “arm switching”. The diverged isomiR expression profiles further enrich the complexity of multiple isomiRs, and complicate the coding-non-coding RNA regulatory network.
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Journal: Gene - Volume 533, Issue 1, 1 January 2014, Pages 149–155