کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2816849 1159955 2013 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A collection of glycosyltransferases from rice (Oryza sativa) exposed to atrazine
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
A collection of glycosyltransferases from rice (Oryza sativa) exposed to atrazine
چکیده انگلیسی


• We identified 59 genes encoding glycosyltransferases (GTs) from rice.
• Many GTs contain diverse cis-elements.
• Most of the encoded GTs contain diverse functional domains.
• These GT genes are located on a wide range of chromosomes.

The rice (Oryza sativa) GTs belong to a super family possibly with hundreds of members. However, which GTs are involved in plant response to toxic chemicals is unknown. Here, we demonstrated 59 novel GT genes screened from our recent genome-wide sequencing datasets of rice crops exposed to atrazine (a herbicide persistent in ecosystems). Analysis of GT genes showed that most of the GTs contain functional domains typically found in proteins transferring glycosyl moieties to their target compounds. A phylogenetic analysis revealed that many GT genes from different families have diverse cis-elements necessary for response to biotic and environmental stresses. Experimental validation for the GTs was undertaken through a microarray, and 36 GT genes were significantly detected with an expression pattern similar to that from deep-sequencing datasets. Furthermore, 12 GT genes were randomly selected and confirmed by quantitative real-time RT-PCR. Finally, the special activity of total GTs was determined in rice roots and shoots, with an increased activity under the atrazine exposure. This response was closely associated with atrazine absorption in the rice tissues. These results indicate that exposure to atrazine can trigger specific GT genes and enzyme activities in rice.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 531, Issue 2, 1 December 2013, Pages 243–252
نویسندگان
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