کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2816940 | 1159959 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cloning and stress-responding expression analysis of malonyl CoA-acyl carrier protein transacylase gene of Nannochloropsis gaditana
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کلمات کلیدی
mCATORFNannochloropsis gaditanaRT-PCRCDD - CSDBLAST, basic local alignment search tool - ابزار پایهای برای جستجوی برهم نهیهای موضعی، بلاستBlast - انفجارFatty acid composition - ترکیب اسید چربreverse transcription PCR - رونویسی معکوس PCRopen reading frame - قاب خواندن بازMolecular weight - وزن مولکولیnon-redundant protein database - پایگاه داده پروتئین غیر انحصاری
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
Malonyl CoA-acyl carrier protein transacylase (MCAT, E2.3.1.39) is closely associated with the FASII pathway of fatty acid biosynthesis. However, the information about microalgal MCAT is scarce. In this study, a MCAT gene was isolated from Nannochloropsis gaditana with its deduced protein (NgMCAT) characterized with bioinformatic tools. The abundance of the NgMCAT transcript under different environmental conditions was determined with real-time quantitative PCR. Results showed that the open reading frame (ORF) of NgMCAT was 1059 bp in length, which encoded 352 amino acid residues. The abundance of NgMCAT transcript reached the maximum (5.17-fold) at 6 h when sodium nitrate was limited, and reached the maximum (4.25-fold) at 12 h at low temperature (15 °C). The abundance of NgMCAT transcript fluctuated at high temperature (35 °C) when the concentration of nitrate and sodium chloride exceeded 150 mg/L and 62 g/L, respectively. In addition, some components of fatty acid that changed with the expression of NgMCAT were also observed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 530, Issue 1, 1 November 2013, Pages 33-38
Journal: Gene - Volume 530, Issue 1, 1 November 2013, Pages 33-38
نویسندگان
Jinhu Tian, Minggang Zheng, Guanpin Yang, Li Zheng, Jiluan Chen, Baijuan Yang,