کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2817230 | 1159976 | 2013 | 9 صفحه PDF | دانلود رایگان |
• Targeted PCR based-procedure for sequencing of large DNA clusters
• Unusual features of a C. jejuni cps gene cluster discovered
• Mechanisms of generation of variant capsular polysaccharides suggested
PCR probing of the genome of Campylobacter jejuni strain X using conserved capsular polysaccharide (CPS)-related genes allowed elucidation of a complete sequence of the respective gene cluster (cps). This is the largest known Campylobacter cps cluster (38 kb excluding flanking kps regions), which includes a number of genes not detected in other Campylobacter strains. Sequence analysis suggests genetic rearrangements both within and outside the cps gene cluster, a mechanism which may be responsible for mosaic organisation of sugar transferase-related genes leading to structural variability of the capsular polysaccharide (CPS).
Journal: Gene - Volume 522, Issue 1, 10 June 2013, Pages 37–45