کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2817353 | 1159982 | 2013 | 6 صفحه PDF | دانلود رایگان |
Local secondary structures in coding sequences have important functions across various translational processes. To date, however, the local structures and their functions in the early stage of translation elongation remain poorly understood. Here, we surveyed the structural stability in the first 180 nucleotides of the coding sequence of 27 species using computational method. We found that the structural stability in the 30–80 nucleotide interval was significantly higher than that in other regions in eukaryotes and most prokaryotes. No significant correlation between local translation efficiency and structural stability was observed, suggesting that this structural region has undergone selection pressure directly to maintain high stability. Furthermore, ribosome was blocked by this region, providing an opportunity for co-translational regulation. Remarkably, in eukaryotes, we found that mRNAs with higher structural stability in the 30–80 nucleotide interval tended to encode the secreted proteins. Overall, our results revealed a previously unappreciated correlation between structural stability and protein localization.
► A high stability region (HSR) on mRNA was found in eukaryotes and prokaryotes.
► HSR has undergone selection pressure to maintain the structural stability.
► HSR slows down the ribosome speed.
► HSR enriches in the genes which encode secreted proteins.
Journal: Gene - Volume 517, Issue 2, 1 April 2013, Pages 230–235