کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2817452 1159989 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
DNA-binding motif and target genes of the imprinted transcription factor PEG3
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
DNA-binding motif and target genes of the imprinted transcription factor PEG3
چکیده انگلیسی

The Peg3 gene is expressed only from the paternally inherited allele located on proximal mouse chromosome 7. The PEG3 protein encoded by this imprinted gene is predicted to bind DNA based on its multiple zinc finger motifs and nuclear localization. In the current study, we demonstrated PEG3's DNA-binding ability by characterizing its binding motif and target genes. We successfully identified target regions bound by PEG3 from mouse brain extracts using chromatin immunoprecipitation analysis. PEG3 was demonstrated to bind these candidate regions through the consensus DNA-binding motif AGTnnCnnnTGGCT. In vitro promoter assays established that PEG3 controls the expression of a given gene through this motif. Consistent with these observations, the transcriptional levels of a subset of the target genes are also affected in a mutant mouse model with reduced levels of PEG3 protein. Overall, these results confirm PEG3 as a DNA-binding protein controlling specific target genes that are involved in distinct cellular functions.


► PEG3 binds specific genomic target regions through its consensus DNA-binding motif AGTnnCnnnTGGCT.
► PEG3 functions as a DNA-binding transcription factor controlling specific cellular functions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 512, Issue 2, 10 January 2013, Pages 314–320
نویسندگان
, , , , , ,