کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2817542 1159994 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Isolation and characterization of a bread wheat salinity responsive ERF transcription factor
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Isolation and characterization of a bread wheat salinity responsive ERF transcription factor
چکیده انگلیسی

A screen conducted on both a suppression subtractive hybridization and a full length cDNA library made from a salinity tolerant bread wheat cultivar SR3 (Triticum aestivum cv. SR3) resulted in the recognition of TaERF4, a gene including both an AP2/ERF domain and a nuclear localization signal. The 982 bp TaERF4 cDNA comprised a 582 bp open reading frame, encoding a 193 residue polypeptide of molecular weight 20 kDa and calculated pI 8.48. A TaERF4-GFP fusion protein localized preferentially to the nuclei of Arabidopsis thaliana protoplasts. TaERF4 is a member of the B-1 group within the ERF sub‐family and was not transactivatable in yeast. The presence of an ERF-associated amphiphilic repression (EAR) motif at its C-terminus suggests that TaERF4 is probably a transcription repressor. TaERF4 was inducible by exposure to salinity and osmotic stresses, but not to exogenously supplied abscisic acid (ABA). The heterologous constitutive expression of TaERF4 in Arabidopsis enhanced the level of sensitivity to salinity stress, possibly via the repression of tonoplast Na+/H+ antiporter activity. There was no phenotype associated with the transgene's presence when plants were subjected to either osmotic stress or ABA treatment. TaERF4 appears to be a transcription repressor acting within the ABA-independent response to salinity stress.


► We cloned a novel ERF gene TaERF4 from salinity tolerant wheat.
► TaERF4 belongs to the B-1 group protein in the ERF subfamily.
► TaERF4 possesses the EAR motif and might be a transcription repressor.
► The expression of TaERF4 was induced by NaCl and PEG but not by ABA.
► Expression of TaERF4 enhanced sensitivity of plants to salinity stress.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 511, Issue 1, 10 December 2012, Pages 38–45
نویسندگان
, , , , , ,