کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2817909 | 1160018 | 2012 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Colocalization and identification of interaction sites between IGFBP-3 and GalNAc-T14 Colocalization and identification of interaction sites between IGFBP-3 and GalNAc-T14](/preview/png/2817909.png)
GalNAc-T14 was identified as a novel IGFBP-3 binding partner in previous studies. Here, we furtherly confirmed the interaction between them by confocal microscopy, and identified the binding domain and probable interaction sites of GalNAc-T14 with IGFBP-3. The result of subcellular localization indicated that GalNAc-T14 was distributed in the cytosol, whereas IGFBP-3 existed in the cytosol and nucleolus. Confocal analyses demonstrated that IGFBP-3 and GalNAc-T14 colocalized in the cytosol. The result from yeast two hybrid assay showed that the C terminus of GalNAc-T14 (408–552aa) was essential for the interaction between GalNAc-T14 and IGFBP-3, especially Tyr408, Pro409, and Glu410 of GalNAc-T14 may play key roles in the interaction with IGFBP-3. In conclusion, these studies demonstrated that IGFBP-3 and GalNAc-T14 are colocalized in MCF-7 cells and confirmed the interaction between IGFBP-3 and GalNAc-T14. This interaction may play an important role in the functional regulation of IGFBP-3.
Journal: Gene - Volume 499, Issue 2, 15 May 2012, Pages 347–351