کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2818074 1160028 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression and characterization of a recombinant Cry1Ac crystal protein fused with an insect-specific neurotoxin ω-ACTX-Hv1a in Bacillus thuringiensis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Expression and characterization of a recombinant Cry1Ac crystal protein fused with an insect-specific neurotoxin ω-ACTX-Hv1a in Bacillus thuringiensis
چکیده انگلیسی

In order to assess possible enhancement of biopesticide activity, the fusion gene of crystal protein gene cry1Ac with the insect-specific neurotoxin ω-ACTX-Hv1a gene and egfp was expressed in Bacillus thuringiensis acrystalliferous strain Cry-B under the control of the native gene expression system. The fusion recombinant Cry-B(1Ac-ACTX-EGFP) generally produced two or three small crystal-like inclusion bodies in each cell and the GFP signal could be clearly observed. A 166 kDa full-length fusion protein was identified by immunoblot analysis. Virulence of the fusion inclusions was at least fivefold higher toward larvae of Spodoptera exigua. These results demonstrated that a foreign protein could be expressed and accumulate as parasporal inclusions in B. thuringiensis by C-terminal fusion with the native endotoxin while retaining partial insecticidal activity.


► The fusion protein Cry1Ac-ω-ACTX-Hv1a-EGFP was successfully expressed in B. t.
► Heterogeneous toxins formed parasporal inclusion bodies together with endotoxins.
► Virulence of the fusion inclusions was significantly improved against S. exigua.
► It is a promising approach for potent biopesticides with lower resistance potential.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 498, Issue 2, 1 May 2012, Pages 323–327
نویسندگان
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