کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2818159 1160034 2012 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterisation of the gene cluster for l-rhamnose catabolism in the yeast Scheffersomyces (Pichia) stipitis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Characterisation of the gene cluster for l-rhamnose catabolism in the yeast Scheffersomyces (Pichia) stipitis
چکیده انگلیسی

In Scheffersomyces (Pichia) stipitis and related fungal species the genes for l-rhamnose catabolism RHA1, LRA2, LRA3 and LRA4 but not LADH are clustered. We find that located next to the cluster is a transcription factor, TRC1, which is conserved among related species. Our transcriptome analysis shows that all the catabolic genes and all genes of the cluster are up-regulated on l-rhamnose. Among genes that were also up-regulated on l-rhamnose were two transcription factors including the TRC1. In addition, in 16 out of the 32 analysed fungal species only RHA1, LRA2 and LRA3 are physically clustered. The clustering of RHA1, LRA3 and TRC1 is also conserved in species not closely related to S. stipitis. Since the LRA4 is often not part of the cluster and it has several paralogues in l-rhamnose utilising yeasts we analysed the function of one of the paralogues, LRA41 by heterologous expression and biochemical characterization. Lra41p has similar catalytic properties as the Lra4p but the transcript was not up-regulated on l-rhamnose. The RHA1, LRA2, LRA4 and LADH genes were previously characterised in S. stipitis. We expressed the l-rhamnonate dehydratase, Lra3p, in Saccharomyces cerevisiae, estimated the kinetic constants of the protein and showed that it indeed has activity with l-rhamnonate.


► The gene cluster for l-rhamnose catabolism includes a transcription factor.
► The cluster genes RHA1, LRA2, LRA3, LRA4 and TRC1 are up-regulated on l-rhamnose.
► The clustering of RHA1, LRA3 and TRC1 is conserved in species distant to S. stipitis.
► The paralogue Lra41p is similar to Lra4p but not up-regulated on l-rhamnose.
► The l-rhamnonate dehydratase, Lra3p, was active when expressed in S. cerevisiae.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 492, Issue 1, 15 January 2012, Pages 177–185
نویسندگان
, , , , , , ,