کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2818431 | 1569859 | 2011 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of genes involved in bamboo fiber development
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کلمات کلیدی
CLSMSSHDEPCConfocal laser scanning microscope (CLSM)SSH library5-bromo-4-chloro-3-indolyl-β-d-galactopyranosidePMTX-GalESTs - EST هاReal time - به موقعFiber development - توسعه فیبرExpressed sequence tags - تگ های توالی بیان شدهdiethylpyrocarbonate - دیاتیلپیر کربناتdigoxigenin - دیگوکسین ژنDIG - شماPhoto Multiplier Tube - عکس چندتاییConfocal laser scanning microscope - میکروسکوپ اسکن لیزر ConfocalIn-situ Hybridization - هیبریداسیون در محلSuppressive subtractive hybridization - هیبریداسیون کم مخلوط
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Recently bamboo has gained reputation as a major resource of non-wood fiber. The present study was undertaken to generate information about fiber development process in bamboo (Bambusa balcooa) using PCR-based suppressive subtractive hybridization (PCR-SSH) technique, as molecular mechanism of its fiber development is yet to be explored. SSH was performed between cDNA isolated from leaf (as driver) and internodes (as tester) of B. balcooa which indicated up-regulation of 521 ESTs. Among these 41 were contigs and 65 ESTs were singletons. On the basis of BLASTX search 307 ESTs with known functions were classified into several functional categories including transport, metabolism, information, perception and response to stimuli while others were either non-significant (120) or hypothetical proteins (94). A total of 51 out of 307 functional ESTs were found fiber specific and their global distribution among different plant species like maize, rice, cotton and Arabidopsis ESTs were determined. Net distributions and differential expression patterns of 13 important B. balcooa fiber specific cDNAs among different internodes during bamboo development were studied using RNA slot-blot, semi-quantitative RT-PCR and real time PCR. In-situ localization of mRNA transcript for few selected bamboo fiber ESTs namely, V1Bb147 (protein kinase-like protein) and V1Bb88 (myb domain-containing protein) were detected using Confocal Laser Scanning Microscope. Transcript levels of these genes exhibited an orchestral turn-over during bamboo development, suggesting their close association with fiber development, an event associated with several metabolic and physiological changes. The results clearly suggest that these genes are involved in several concerted mechanisms involving Ca+ signaling pathway, cell wall synthesis, hormone regulation, system maintaining cell turgor pressure and cytoskeleton synthesis pathway accountable for bamboo fiber development signifying fiber development as a complex but ordered metabolic process involving differential expression of large scale fiber associated genes. This is the first report on systematic analysis of genes involved in bamboo fiber development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 478, Issues 1â2, 1 June 2011, Pages 19-27
Journal: Gene - Volume 478, Issues 1â2, 1 June 2011, Pages 19-27
نویسندگان
Vineeta Rai, Jayadri Sekhar Ghosh, Amita Pal, Nrisingha Dey,