کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2818458 1569863 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Translation efficiencies of synonymous codons for arginine differ dramatically and are not correlated with codon usage in chloroplasts
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Translation efficiencies of synonymous codons for arginine differ dramatically and are not correlated with codon usage in chloroplasts
چکیده انگلیسی

Codon usage in chloroplast mRNAs is different from that in prokaryotic and cytosolic mRNAs. We previously devised an in vitro assay for translation efficiencies using synthetic mRNAs, and measured translation efficiencies of five synonymous codon groups in tobacco chloroplasts. Using this assay, we here report our analysis of four additional synonymous codon groups in tobacco chloroplasts. We found that translation efficiencies of three arginine codons AGA, CGU and CGA differ dramatically, ca. 10-fold difference although the three arginine codons possess similar codon usage. Translation of AGA is very high, while CGA is translated extremely low. CGA is used frequently in chloroplasts but rare in Escherichia coli. The single tRNA species reads two histidine codons (CAU and CAC) and this is also the case for two glutamic acid codons (GAA and GAG) and two arginine codons (GCU and GCA). Their translation efficiencies, however, differ significantly. These observations suggest that individual codons posses their intrinsic efficiencies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 472, Issues 1–2, 1 February 2011, Pages 50–54
نویسندگان
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