کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2818568 1569870 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A versatile set of tagged expression vectors to monitor protein localisation and function in Dictyostelium
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
A versatile set of tagged expression vectors to monitor protein localisation and function in Dictyostelium
چکیده انگلیسی

We describe here a series of vectors for ectopic expression of tagged proteins in Dictyostelium discoideum. These vectors allow the addition of N- or C-terminal tags (GFP, mRFP, 3xFLAG, 3xHA, 6xMYC or TAP) with an optimised polylinker sequence and no additional amino acid residues at the N- or C-terminus of the protein. The expression cassettes were introduced into vectors containing Blasticidin or Geneticin resistance markers and into integrating as well as extrachromosomal plasmids. The vectors are designed as high and low copy versions and thus allow for a limited expression level control. They are also convenient with regard to complementation, co- and super-transformation. Finally the vectors share standardised cloning sites, so that a gene of interest can be easily transferred between vectors as experimental requirements evolve. These vectors were used to study the localisation of several putative RNA processing proteins including EriA and DicerB.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 465, Issues 1–2, 1 October 2010, Pages 1–8
نویسندگان
, ,