کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2818984 | 1569895 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid proteasomal degradation of transcription factor IIB in accordance with F9 cell differentiation
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کلمات کلیدی
GTF, general transcription factorCHX, cycloheximide - CHX، cycloheximideRA, retinoic acid - RA، اسید رتینوئیکRNAPII, RNA polymerase II - RNAPII، RNA پلیمراز IISDS-PAGE, SDS-Polyacrylamide Gel Electrophoresis - SDS-PAGE، الکتروفورز ژل SDS-polyacrylamide gelTBP, TATA-binding protein - TBP، پروتئین TATA اتصال
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Rapid proteasomal degradation of transcription factor IIB in accordance with F9 cell differentiation Rapid proteasomal degradation of transcription factor IIB in accordance with F9 cell differentiation](/preview/png/2818984.png)
چکیده انگلیسی
We found that the levels of all general transcription factors (GTFs) for RNA polymerase II decreased in F9 cells when the cells were subjected to a differentiation procedure. Different from other GTFs, decrease of TFIIB during the differentiation was suppressed by addition of a proteasome inhibitor, MG132. The half-life of TFIIB in the differentiated cells was remarkably reduced compared with that in the undifferentiated cells. Moreover, it was demonstrated that TFIIB is a poly-ubiquitinated protein. Results of this study suggest that components of the transcription machinery decreased in accordance with cell differentiation and that TFIIB is specifically and rapidly degraded by the ubiquitin–proteasome pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 436, Issues 1–2, 1 May 2009, Pages 115–120
Journal: Gene - Volume 436, Issues 1–2, 1 May 2009, Pages 115–120
نویسندگان
Seiji Shiraishi, Naomi Tamamura, Misako Jogo, Yuji Tanaka, Taka-aki Tamura,