کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2819739 | 1160149 | 2007 | 10 صفحه PDF | دانلود رایگان |
Alteration of gene expression is a crucial component of adaptation by animals to hypoxic conditions and is mediated by specific transcription factors, hypoxia-inducible factors (HIFs), which are composed of α and β subunits. In this study, we report the cloning and characterization of two HIF-α subunits, HIF-1α and HIF-2α, and their expression in various tissues of a hypoxia-tolerant marine teleost, Atlantic croaker (Micropogonias undulatus). The full-length croaker HIF-1α (2805 bp) and HIF-2α (3205 bp) cDNAs contain open reading frames encoding proteins with 720 and 847 amino acids, respectively, which are highly homologous to the HIF-1α and HIF-2α proteins of other non-mammalian species. Croaker HIF-1α shares only 43% sequence identity with the croaker HIF-2α subunit. However, the basic helix–loop–helix/Per–ARNT–Sim regions appear to be relatively well conserved between the two proteins, with identities of 75–83%. The core oxygen-dependent degradation domain regions in croaker HIFs are well conserved, suggesting a similar mechanism of HIF degradation to that in other vertebrate species. Northern blot analysis showed that croaker HIF-1α and HIF-2α mRNAs (transcript sizes 3.0–3.8 kb) are highly expressed in the brain, heart, liver, and gonads under hypoxic conditions, whereas muscle tissues show lower levels of expression. Short-term (1.7 mg/L dissolved oxygen, DO for 3 days to 1 week) and long-term (1.7, 2.7 and 3.7 mg/L DO for 3 weeks) hypoxia exposure caused significant increases in HIF-1α and HIF-2α mRNA expression in croaker ovaries compared to mRNA levels in fish held in normoxic conditions (DO: 6.5 mg/L). However, HIF transcript levels in hypoxia-exposed fish had returned to control values 24 h after the DO in the tanks was restored to normoxic levels. The results suggest that the upregulation of both HIF-1α and HIF-2α subunits at the transcriptional level is an important component of adaptation of croaker to chronic hypoxia and HIF-αs are potentially useful molecular indicators of environmental hypoxia exposure.
Journal: Gene - Volume 396, Issue 2, 15 July 2007, Pages 273–282