کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2819901 | 1569939 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cloning, characterization, expression and comparative analysis of pig Golgi membrane sphingomyelin synthase 1
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Cloning, characterization, expression and comparative analysis of pig Golgi membrane sphingomyelin synthase 1 Cloning, characterization, expression and comparative analysis of pig Golgi membrane sphingomyelin synthase 1](/preview/png/2819901.png)
چکیده انگلیسی
Pig sphingomyelin synthase 1 (SMS1) cDNA was cloned, characterized and compared to the human ortholog. Porcine protein consists of 413 amino acids and displays a 97% sequence identity with human protein. A phylogenic tree of proteins reveals that porcine SMS1 is more closely related to bovine and rodent proteins than to human. Analysis of protein mass was higher than the theoretical prediction based on amino acid sequence suggesting a kind of posttranslational modification. Quantitative representation of tissue distribution obtained by real-time RT-PCR showed that it was widely expressed although important variations in levels were obtained among organs. Thus, the cardiovascular system, especially the heart, showed the highest value of all the tissues studied. Regional differences of expression were observed in the central nervous system and intestinal tract. Analysis of the hepatic mRNA and protein expressions of SMS1 following turpentine treatment revealed a progressive decrease in the former paralleled by a decrease in the protein concentration. These findings indicate the variation in expression in the different tissues might suggest a different requirement of Golgi sphingomyelin for the specific function in each organ and a regulation of the enzyme in response to turpentine-induced hepatic injury.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 388, Issues 1â2, 15 February 2007, Pages 117-124
Journal: Gene - Volume 388, Issues 1â2, 15 February 2007, Pages 117-124
نویسندگان
Natalia Guillén, MarÃa A. Navarro, JoaquÃn C. Surra, Carmen Arnal, Marta Fernández-Juan, Jose Alvaro Cebrián-Pérez, Jesús Osada,