کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2820057 1569947 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Similarity and divergence between the RNA polymerase α subunits from hyperthermophilic Thermotoga maritima and mesophilic Escherichia coli bacteria
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Similarity and divergence between the RNA polymerase α subunits from hyperthermophilic Thermotoga maritima and mesophilic Escherichia coli bacteria
چکیده انگلیسی

The α subunit (αTm) of Thermotoga maritima RNA polymerase has been characterized to investigate its role in transcriptional regulation in one of the few known anaerobic hyperthermophilic bacteria. The highly thermostable αTm shares 54% similarity with its Escherichia coli analogue (αEc). The T. maritima rpoA gene coding the α subunit does not complement the thermosensitive rpoA112 mutation of E. coli. However, αTm and αEc show similar folding patterns as determined by circular dichroism. Purified αTm binds to the T. maritima PargGo promoter region (probably to a UP-element) and Arg282 appears to be crucial for DNA binding. The thermostable protein is also able to interact with transcription regulatory proteins, like ArgR from T. neapolitana or CRP from E. coli. These data indicate that the RNA polymerase α subunit might play a crucial role in the modulation of gene expression in hyperthermophiles.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 380, Issue 2, 1 October 2006, Pages 120–126
نویسندگان
, , , , , , ,