کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2836196 | 1570845 | 2016 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Characterization of novel type I ribosome-inactivating proteins isolated from oil palm (Elaeis guineensis) inoculated with Ganoderma boninense, the causal agent of basal stem rot Characterization of novel type I ribosome-inactivating proteins isolated from oil palm (Elaeis guineensis) inoculated with Ganoderma boninense, the causal agent of basal stem rot](/preview/png/2836196.png)
• Novel type I RIPs were detected in oil palm.
• EgRIPs expression levels were altered during plant-pathogen interaction.
• EgRIPs demonstrated depurination activity.
• EgRIPs inhibited Ganoderma boninense mycelial growth in vitro.
Type I RIPs have not been identified in oil palm. EgRIP-1a and EgRIP-1b transcripts were partially isolated from roots and basal stems of oil palm seedlings with high similarity to type I RIPs. Expression levels were altered during plant-pathogen interaction and their proteins were 28–30 kD with an estimated pI value of 10.0. Depurination of yeast 26S rRNA by EgRIP proteins demonstrated specific ribosome-inactivating activity. EgRIP proteins showed inhibition on G. boninense mycelial growth with 44.1% at 5 DAI. It was concluded that the novel EgRIPs are type I and demonstrated to have antifungal effect against G. boninense.
Journal: Physiological and Molecular Plant Pathology - Volume 94, April 2016, Pages 53–61