Pseudomonas putida BP25 alters root phenotype and triggers salicylic acid signaling as a feedback loop in regulating endophytic colonization in Arabidopsis thaliana

• Pseudomonas putida BP25 inflicted phenotypic alterations in roots of Arabidopsis thaliana Col-0 upon its colonization.
• Endogenous population size of P. putida BP25 was found internally regulated in A. thaliana Col-0.
• P. putida BP25 induced Salicylic Acid mediated defense genes.
• Genes participating in growth and developmental processes in A. thaliana Col-0 were found repressed.

Endophytic Pseudomonas putida BP25 (PpBP25) triggered density dependent alterations on Arabidopsis thaliana Col-0 growth. Endogenous colonization of PpBP25 was found regulated within Arabidopsis that caused induction and repression of 131 and 74 plant genes, respectively. Induced genes like WRKY33, AtRLP19, ATL2, ATEXO70B2, pEARLI, RPS2, CBP60G, PLA2, CRK18, ATFBS1, DREB2A, TIR, RAP2.4, and MOS1 were components of defense and salicylic acid (SA) signaling. Development associated genes were found significantly repressed. Biased activation of phytohormone signaling with their associated fitness costs on plant growth was observed. The data suggests that PpBP25 colonization triggered expression of defense genes that restricted its own population in a feedback loop besides causing altered root phenotype.

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