کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2840533 1165330 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of three pheromone-binding proteins (PBPs) of Helicoverpa armigera (Hübner) and their binding properties
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش حشره شناسی
پیش نمایش صفحه اول مقاله
Characterization of three pheromone-binding proteins (PBPs) of Helicoverpa armigera (Hübner) and their binding properties
چکیده انگلیسی

Three pheromone-binding proteins of Helicoverpa armigera were cloned and expressed in Escherichia coli. In order to characterize their physiological properties, ligand-binding experiments were performed using five biologically relevant substances including sex pheromones and interspecific signals. The results showed that one of the pheromone-binding proteins, HarmPBP1, binds strongly to each of the two principal pheromone components of H. armigera, (Z)-11-tetradecenal and (Z)-9-hexadecenal, but not to the interspecific signal (Z)-9-tetracecenal. The two remaining pheromone-binding proteins, HarmPBP2 and HarmPBP3, showed only weak affinities with the ligands tested. The 3-D structure of HarmPBP1 was predicted and the docking experiments indicate that the key binding site of (Z)-9-hexadecenal to HarmPBP1 includes Thr112, Lys111, and Phe119 whereas that of (Z)-11-tetradecenal includes Ser9, Trp37, Phe36, and Phe119.

Pheromone binding proteins of Helicoverpa armigera were binding with two sex pheromone components (Z) 11-tetradecenal and (Z) 9-hexadecenal, and some interspecific signals.Figure optionsDownload as PowerPoint slideHighlights
► We get three PBPs from the cDNA library of the antennae of Helicoverpa armigera.
► Analyzed the three PBP genes with other Lepidoptera.
► Expressed the three PBPs in E. coli and purified them.
► Tested five sex pheromones with each PBP by binding experiment.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Insect Physiology - Volume 58, Issue 7, July 2012, Pages 941–948
نویسندگان
, , , , , ,