کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2840565 | 1165332 | 2013 | 7 صفحه PDF | دانلود رایگان |
• Photoperiod-sensitive stage in Helicoverpa armigera larvae for diapause induction is the fifth to the early sixth instar.
• PP2A-Aα was identified by SSH as an up-regulated gene during the photoperiod-sensitive stage.
• High expression of PP2A-Aα was observed during the photoperiod-sensitive stage of diapause-destined individuals and correlates with diapause induction.
Protein phosphatase 2A (PP2A) is a major serine-threonine protein phosphatase which regulates metabolism, transcription, RNA splicing, translation, differentiation, cell cycle, oncogenic transformation and signal transduction. PP2A-Aα, an isoform of PP2A-A, is a structural subunit of the PP2A complex. We identified the photoperiod-sensitive stage for pupal diapause induction to be from the fifth instar to the early sixth instar larvae in the cotton bollworm, Helicoverpa armigera. PP2A-Aα cDNA from brains of diapause-destined fifth instar larvae was obtained by suppressive subtractive hybridization using nondiapause-destined larval brains as a control. Developmental expression of PP2A-Aα mRNA during the photoperiod-sensitive stage was higher in brains of diapause-destined larvae, and the PP2A-Aα protein showed a similar expression pattern as the mRNA. When larvae were transferred from diapause-inducing short days to long days during the diapause-sensitive stage, both PP2A-Aα mRNA and protein decreased significantly, and diapause incidence was also reduced. Thus, high PP2A-Aα expression during the diapause-sensitive stage may play a crucial role in photoperiodic induction of diapause, suggesting that it may be a new player involved in the molecular mechanism for diapause induction.
High expression of PP2A-A was observed during the photoperiod-sensitive stage of diapause-destined individuals and correlates with diapause induction.Figure optionsDownload as PowerPoint slide
Journal: Journal of Insect Physiology - Volume 59, Issue 6, June 2013, Pages 588–594