High affinity 3H-phenylalanine uptake by brush border membrane vesicles from whole larvae of Aedes aegypti (AaBBMVw)

► AaBBMVw are valid for studying characteristics of nutrient amino acid transporters. ► The Phe concentration in the lumen of 4th instar Anopheles gambiae midgut is 0.1 μM. ► The [H+], the K0.5 for Phe±, Na+ and K+ and for 3H-Phe uptake are all ⩽1 μM. ► Concentrations can be set in AaBBMVw that are in their native lipid backgrounds. ► AaBBMVw can be prepared from >300,000 larvae, frozen and shipped anywhere.