کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2841203 | 1165383 | 2008 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Importance of molecular configuration in gap junctional permeability
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش حشره شناسی
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چکیده انگلیسی
Gap junctions between insect oocytes and follicular epithelial cells allow transit of elongate Ca2+-binding proteins Calmodulin (CaM, 17Â kDa) and Troponin-C (Trop-C, 18Â kDa), but not multi-branched dextran (10Â kDa) nor the Ca2+-binding protein Osteocalcin (Osteo, 6Â kDa). By microinjection of fluorescently labeled versions of each of these molecules we were able to obtain visual evidence that, despite their lesser molecular weight, molecules with greater cross-sections were unable to transit these gap junctions, while heavier but elongate molecules could. While CaM had previously been shown to pass through gap junctions from oocytes to their surrounding epithelial cells, the ability of CaM and Trop-C to transit the gap junctions between adjacent epithelial cells had not been demonstrated. Evidence shown here demonstrates that the homologous gap junctions among epithelial cells, like the heterologous gap junctions between epithelial cells and the oocyte they surround, allow transit of elongate molecules up to at least 18Â kDa. Furthermore, the evidence for four different molecules of differing molecular weights and configurations supports the hypothesis that it is molecular configuration, not chemical activity, that primarily determines the observed permeability of gap junctions to molecules 5-6 times larger than the molecular weight limit previously acknowledged.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Insect Physiology - Volume 54, Issue 8, August 2008, Pages 1293-1300
Journal: Journal of Insect Physiology - Volume 54, Issue 8, August 2008, Pages 1293-1300
نویسندگان
Anne M. Cieniewicz, Richard I. Woodruff,