کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2841294 1165388 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Transduction pathways mediated by second messengers including cAMP in the sugar receptor cell of the blow fly: Study by the whole cell clamp method
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش حشره شناسی
پیش نمایش صفحه اول مقاله
Transduction pathways mediated by second messengers including cAMP in the sugar receptor cell of the blow fly: Study by the whole cell clamp method
چکیده انگلیسی

The whole cell clamp method was directly applied to the sensory receptor neurons isolated from the adult labellar hair of the blow fly Phormia regina to locate the signal transduction pathways mediated by second messengers. First, the cAMP-mediated transduction pathway was examined to specify its location in the sugar receptor cell. Sugar receptor cell was identified by recording inward current flow under the voltage clamp applying sucrose solution to the surface of the taste neurons. When cyclic nucleotides, such as cGMP and cAMP, were introduced into the sugar receptor cell, inward current was observed (cGMP, 70 pA; cAMP, 300 pA at 350 μM). Inhibitors and activators for the second messengers (GDPβS and forskolin) and non-cyclic nucleotides were also examined. Second, non-nucleotide second messengers (IP3 and Ca2+) were examined. The sugar receptor cell was activated when it was injected with IP3 or Ca2+. All the obtained results suggest that the cAMP-mediated signal transduction pathway plays a major role in the sugar receptor cell. The possibility of other transduction pathways mediated by IP3 or Ca2+ was not excluded.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Insect Physiology - Volume 54, Issue 6, June 2008, Pages 1028–1034
نویسندگان
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