کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2846651 1571300 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interleukin-1β and interleukin-6 enhance thermal prolongation of the LCR in decerebrate piglets
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی فیزیولوژی
پیش نمایش صفحه اول مقاله
Interleukin-1β and interleukin-6 enhance thermal prolongation of the LCR in decerebrate piglets
چکیده انگلیسی


• Thermal stress and prior upper respiratory tract infection are both risk factors for SIDS.
• Elevated body temperature and Il-1β and IL-6, the likely mediators of thermal stress and adverse effects of prior infection, both prolong the laryngeal chemoreflex in neonatal decerebrate piglets.
• Thermal prolongation of the laryngeal chemoreflex and cytokine-mediated prolongation of the laryngeal chemoreflex interact to amplify the effect of each other and markedly prolong the laryngeal chemoreflex.
• Both cytokine-mediated and thermal prolongation are likely to be mediated through TRPV1 receptors expressed presynaptically on C-fiber afferents that, in part, mediate the laryngeal chemoreflex.

Thermal stress and prior upper respiratory tract infection are risk factors for the Sudden Infant Death Syndrome. The adverse effects of prior infection are likely mediated by interleukin-1β (IL-1β). Therefore, we examined the single and combined effects of IL-1β and elevated body temperature on the duration of the Laryngeal Chemoreflex (LCR) in decerebrate neonatal piglets ranging in age from post-natal day (P) 3 to P7. We examined the effects of intraperitoneal (I.P.) injections of 0.3 mg/Kg IL-1β with or without I.P. 10 mg/Kg indomethacin pretreatment on the duration of the LCR, and in the same animals we also examined the duration of the LCR when body temperature was elevated approximately 2 °C. We found that IL-1β significantly increased the duration of the LCR even when body temperature was held constant. There was a significant multiplicative effect when elevated body temperature was combined with IL-1β treatment: prolongation of the LCR was significantly greater than the sum of independent thermal and IL-1β-induced prolongations of the LCR. The effects of IL-1β, but not elevated body temperature, were blocked by pretreatment with indomethacin alone. We also tested the interaction between IL-6 given directly into the nucleus of the solitary tract (NTS) bilaterally in 100 ngm microinjections of 50 μL and pretreatment with indomethacin. Here again, there was a multiplicative effect of IL-6 treatment and elevated body temperature, which significantly prolonged the LCR. The effect of IL-6 on the LCR, but not elevated body temperature, was blocked by pretreatment with indomethacin. We conclude that cytokines interact with elevated body temperature, probably through direct thermal effects on TRPV1 receptors expressed pre-synaptically in the NTS and through cytokine-dependent sensitization of the TRPV1 receptor. This sensitization is likely initiated by cyclo-oxygenase-2 dependent synthesis of prostaglandin E2, which is stimulated by elevated levels of IL-1β or IL-6. Inflammatory sensitization of the LCR coupled with thermal prolongation of the LCR may increase the propensity for apnea and Sudden Infant Death Syndrome.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Respiratory Physiology & Neurobiology - Volume 230, August 2016, Pages 44–53
نویسندگان
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