کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2846709 | 1571304 | 2016 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Respiratory calcium fluctuations in low-frequency oscillating astrocytes in the pre-Bötzinger complex Respiratory calcium fluctuations in low-frequency oscillating astrocytes in the pre-Bötzinger complex](/preview/png/2846709.png)
• Pre-Bötzinger astrocytes exhibit synchronized low-frequency calcium oscillations.
• Calcium signals in 10% of astrocytes precede inspiratory neuronal activity.
• Astrocytic signals may contribute to variability of the respiratory rhythm.
Astrocytes have been found to modulate neuronal activity through calcium-dependent signaling in various brain regions. However, whether astrocytes of the pre-Bötzinger complex (preBötC) exhibit respiratory rhythmic fluctuations is still controversial. Here we evaluated calcium-imaging experiments within preBötC in rhythmically active medullary slices from TgN(hGFAP-EGFP) mice using advanced analyses. 13.8% of EGFP-negative cells, putative neurons, showed rhythmic fluorescent changes that were highly correlated to the respiratory rhythmic fluctuation (cross-correlation coefficient > 0.5 and dF/F > 0.2%). In contrast, a considerable number of astrocyte somata exhibited synchronized low-frequency (<0.03 Hz) calcium oscillations. After band-pass filtering, signals that irregularly preceded the calcium signal of EGFP-negative cells were observed in 10.2% of astrocytes, indicating a functional coupling between astrocytes and neurons in preBötC. A model simulation confirmed that such preinspiratory astrocytic signals can arise from coupled neuronal and astrocytic oscillators, supporting a concept that slow oscillatory changes of astrocytic functions modulate neighboring neuronal activity to add variability in respiratory rhythm.
Journal: Respiratory Physiology & Neurobiology - Volume 226, June 2016, Pages 11–17