کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2847121 1571338 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Noninvasive assessment of alveolar microvascular recruitment in conscious non-sedated rats
ترجمه فارسی عنوان
ارزیابی غیرتهاجمی از استخراج میکروواسکولار آلوئولار در موش های صحرایی آگاه نشده
کلمات کلیدی
ظرفیت پخش ریه، جریان خون ریوی، چاقی، دیابت نوع 2، سندرم متابولیک، میکروآنژیوپاتی آلوئولار
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی فیزیولوژی
چکیده انگلیسی


• A noninvasive rebreathing method is developed to measure lung function in conscious rats.
• Alveolar microvascular reserves are assessed from the pulmonary diffusing capacity-to-perfusion relationship.
• Small animals show reduced microvascular reserves compared to large species.
• Rats with metabolic syndrome show greater age-exacerbated loss of alveolar microvascular reserves.

Recruitment of alveolar microvascular reserves, assessed from the relationship between pulmonary diffusing capacity (DLCO) and perfusion (Q˙c), is critical to the maintenance of arterial blood oxygenation. Leptin-resistant ZDF fatty diabetic (fa/fa) rats exhibit restricted cardiopulmonary physiology under anesthesia. To assess alveolar microvascular function in conscious, non-sedated, non-instrumented, and minimally restrained animals, we adapted a rebreathing technique to study fa/fa and control non-diabetic (+/+) rats (4–5 and 7–11 mo old) at rest and during mild spontaneous activity. Measurements included O2 uptake, lung volume, Q˙c, DLCO, membrane diffusing capacity (DMCO), capillary blood volume (Vc) and septal tissue-blood volume. In older fa/fa than +/+ animals, DLCO and DMCO at a given Q˙c were lower; Vc was reduced in proportion to Q˙c. Results demonstrate the consequences of alveolar microangiopathy in the metabolic syndrome: lung volume restriction, reduced Q˙c, and elevated membrane resistance to diffusion. At a given Q˙c, DLCO is lower in rats and guinea pigs than dogs or humans, consistent with limited alveolar microvascular reserves in small animals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Respiratory Physiology & Neurobiology - Volume 190, 1 January 2014, Pages 105–112
نویسندگان
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