کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2848097 1167405 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Role of glutamate and substance P in the amphibian respiratory network during development
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی فیزیولوژی
پیش نمایش صفحه اول مقاله
Role of glutamate and substance P in the amphibian respiratory network during development
چکیده انگلیسی
This study tested the hypothesis that glutamatergic ionotropic (AMPA/kainate) receptors and neurokinin receptors (NKR) are important in the regulation of respiratory motor output during development in the bullfrog. The roles of these receptors were studied with in vitro brainstem preparations from pre-metamorphic tadpoles and post-metamorphic frogs. Brainstems were superfused with an artificial cerebrospinal fluid at 20-22 °C containing CNQX, a selective non-NMDA antagonist, or with substance P (SP), an agonist of NKR. Blockade of glutamate receptors with CNQX in both groups caused a reduction of lung burst frequency that was reversibly abolished at 5 μM (P < 0.01). CNQX, but not SP, application produced a significant increase (P < 0.05) in gill and buccal frequency in tadpoles and frogs, respectively. SP caused a significant increase (P < 0.05) in lung burst frequency at 5 μM in both groups. These results suggest that glutamatergic activation of AMPA/kainate receptors is necessary for generation of lung burst activity and that SP is an excitatory neurotransmitter for lung burst frequency generation. Both glutamate and SP provide excitatory input for lung burst generation throughout the aquatic to terrestrial developmental transition in bullfrogs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Respiratory Physiology & Neurobiology - Volume 162, Issue 1, 30 June 2008, Pages 24-31
نویسندگان
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