کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2848331 1571352 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Changes in glucose do not alter baseline firing rate or chemosensitivity of serotonin neurons cultured from the medullary raphé
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی فیزیولوژی
پیش نمایش صفحه اول مقاله
Changes in glucose do not alter baseline firing rate or chemosensitivity of serotonin neurons cultured from the medullary raphé
چکیده انگلیسی

A subset of serotonin neurons are putative central respiratory chemoreceptors. To test the hypothesis that serotonin neurons also have intrinsic glucose sensitivity, perforated patch recordings were made from cultured rat medullary raphé neurons after pharmacological blockade of fast glutamatergic and GABAergic synaptic transmission. It has previously been shown that all neurons stimulated by acidosis under these conditions are tryptophan hydroxylase (TPH) immunoreactive. Changes in glucose concentration from 0.1 to 20 mM had no effect on baseline firing rate of 31 neurons that were stimulated by hypercapnic acidosis. The response to hypercapnic acidosis of 25 of these neurons was the same in 0.1, 1, 2, 5 and/or 20 mM glucose as it was in 10 mM glucose. Changes in glucose also did not alter the baseline firing rate of 13 neurons that did not respond to acidosis. Although it is possible that these results were influenced by the use of tissue culture, they do not provide support for intrinsic glucose sensitivity of pH-sensitive serotonin neurons of the medullary raphé.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Respiratory Physiology & Neurobiology - Volume 157, Issues 2–3, 1 August 2007, Pages 235–241
نویسندگان
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