کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
32845 44945 2009 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A reversible surface functionalized nanowire transistor to study protein–protein interactions
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
A reversible surface functionalized nanowire transistor to study protein–protein interactions
چکیده انگلیسی

SummaryTaking advantage of the reversible association–dissociation between glutathione (GSH) and glutathione S-transferase (GST), we have developed a novel method by surface-functionalizing GSH on silicon nanowire field-effect transistors (SiNW-FET) and then anchoring a particular GST-fused protein for the studies of protein–protein interactions. The reversible surface functionalization method has been proven by analytical techniques, fluorescence imaging, atomic force microscopic imaging, and electrical measurements. The reversible GSH–GST functionality on the SiNW-FET has made this sensorial device consecutively reusable and calibratable, allowing for quantitative analysis. In this work, we have demonstrated that the biologically modified SiNW-FET can be used as a screening sensor for the future studies of biomolecular associations, such as protein–protein interactions, protein–DNA interactions, protein–carbohydrate interactions, etc.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 4, Issue 3, June 2009, Pages 235–243
نویسندگان
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