کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
415300 681197 2008 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparisons of titer estimation methods for multiplexed pneumococcal opsonophagocytic killing assay
موضوعات مرتبط
مهندسی و علوم پایه مهندسی کامپیوتر نظریه محاسباتی و ریاضیات
پیش نمایش صفحه اول مقاله
Comparisons of titer estimation methods for multiplexed pneumococcal opsonophagocytic killing assay
چکیده انگلیسی

Titer estimation is one of the major components of immunoassay and vaccine development. A multiplexed in vitro opsonization assay (MOPA) is widely accepted to quantitate Streptococcus pneumococcal antibodies to serotype-specific pneumococcal capsular polysaccharide. Titer estimation of vaccine based on OPA is one important component of standardization of OPA, and the selected statistical method is a factor influencing the accuracy and precision of titer estimation. We evaluated five titer estimation methods for pneumococcal OPA in terms of precision and accuracy using three data sets generated by specifically designed experiments with both an eight-dilution and an eleven-dilution design. The bootstrap resampling technique was also used to determine the performance of the estimation. We concluded that the traditional direct method did not perform as well as the other four methods in terms of precision and accuracy of titer estimation. The Spearman–Kärber estimator might be biased upward for OPA titer estimation. The four-parameter logistic model (4PL) method is an alternative choice for OPA titer estimation. The eleven-dilution design provided more information than the eight-dilution design for titer estimation and enhanced precision of estimators. UAB opsotiter, computer software using the statistical language R and Microsoft Excel®, was developed to implement OPA titer estimation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Computational Statistics & Data Analysis - Volume 52, Issue 11, 15 July 2008, Pages 5022–5032
نویسندگان
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