کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
429432 687553 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Combinatorial selection of short triplex forming oligonucleotides for fluorescence in situ hybridisation COMBO-FISH
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی کامپیوتر نظریه محاسباتی و ریاضیات
پیش نمایش صفحه اول مقاله
Combinatorial selection of short triplex forming oligonucleotides for fluorescence in situ hybridisation COMBO-FISH
چکیده انگلیسی

For the selection of short triplex forming oligonucleotides for COMBO-FISH hybridisations used in research and medicine for 3D-structure investigations of the nucleus, the human genome has to be scanned for polypurine or poly-pyrimidine sequences which colocalise at the desired genomic region. Further binding sites of the selected oligonucleotides are required not to cluster anywhere else. We present an implementation of algorithms which design such oligonucleotide sets and exemplify the existence of COMBO-FISH probe sets by designing labelling sets for 29 genes and subsets thereof. The algorithms can be trivially parallelised and run on clusters, grids, and clouds.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Computational Science - Volume 3, Issue 5, September 2012, Pages 328–334
نویسندگان
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