Listeria ivanovii ATCC 19119 strain behaviour is modulated by iron and acid stress

• Listeria ivanovii (ATCC 19119 strain) shows a different behaviour compared to Listeria monocytogenes.
• In different iron and pH conditions L. ivanovii (ATCC 19119 strain) doesn't mount an ATR.
• Iron excess and acidic pH induce the highest bacterial invasion in Caco-2 cells.
• Low iron and acidic conditions improve invasion ability in amniotic WISH cells.

It has been suggested that the rarity of human listeriosis due to Listeria ivanovii reflects not only host tropism factors but also the rare occurrence of this species in the environment, compared with Listeria monocytogenes. In the present study we evaluate the effects on the reference strain L. ivanovii ATCC 19119 behaviour of two combined stresses, low iron availability and acid environment, that bacteria can encounter in the passage from saprophytic life to the host. In these conditions, L. ivanovii evidenced a different behaviour compared to L. monocytogenes exposed to similar conditions. L. ivanovii was not able to mount an acid tolerance response (ATR) even if, upon entry into the stationary phase in iron-loaded medium, growth phase-dependent acid resistance (AR) was evidenced. Moreover, bacteria grown in iron excess and acidic pH showed the higher invasion value in Caco-2 cells, even though it was not able to efficiently multiply. On the contrary, low iron and acidic conditions improved invasion ability in amniotic WISH cells.