کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4363666 1301569 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fluorescent in situ hybridization in combination with filter cultivation (FISHFC) method for specific detection and enumeration of viable Clostridium perfringens
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Fluorescent in situ hybridization in combination with filter cultivation (FISHFC) method for specific detection and enumeration of viable Clostridium perfringens
چکیده انگلیسی

To reduce time for enumeration of viable Clostridium perfringens, fluorescence in situ hybridization in combination with filter cultivation (FISHFC) was employed. The method utilized a CLP-180 probe, based on the 16S rRNA region of C. perfringens, and FISHFC fluorescence microscopy to detect C. perfringens, but not organisms from other species. Optimal cultivation requirements for micro-colony formation were TSC medium, anaerobic conditions, 37 °C, and incubation for 6 h. Under these conditions, micro-colony diameters reached 100 μm, a size sufficient for hybridization. Enumeration of C. perfringens using the CLP-180-aided FISHFC method was realized in 9 h as compared to 3–5 days required by the conventional plate count method. Moreover, viable C. perfringens counts of food samples from the two methods were not significantly different.It was concluded that the CLP-180-aided FISHFC is faster than conventional plate count methods and equally accurate (detection limit: 2 log CFU/g). The CLP-180-aided FISHFC method for rapidly evaluating C. perfringens in food samples, can contribute to ensuring food safety.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Microbiology - Volume 26, Issue 4, June 2009, Pages 425–431
نویسندگان
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