کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4382092 1617797 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bacterial community structure in maize stubble-amended soils with different moisture levels estimated by bar-coded pyrosequencing
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
Bacterial community structure in maize stubble-amended soils with different moisture levels estimated by bar-coded pyrosequencing
چکیده انگلیسی


• Long-term straw amendment lessens the difference in bacterial community structure between the unfertilized soil and successive organic manure-fertilized soil.
• Moisture strongly affects bacterial distribution in the stubble-amended soils.
• Soil DOC, which drives the alternative dominance of two functional groups (copiotrophic and oligotrophic bacteria), is a critical property in shaping bacterial community composition in the amended soils.

It is of ecological significance to investigate microbial communities in response to straw amendment and moisture in arable soils. However, in Chinese fluvo-aquic soils, these responses are still poorly understood. We designed an incubation experiment involving two soils with and without the addition of maize stubble at two moisture levels, and bacterial community structure at days 20, 80, and 200 after the start of incubation was assessed via bar-coded pyrosequencing of the 16S rDNA amplicons. In the presence of stubble with identical moisture level, we observed higher bacterial diversity and richness in long-term organic manure-fertilized soil compared with the unfertilized soil at days 20 and 80, which we attributed to the different quality and quantity of organic matter between the two soils. However, there was no significant difference in bacterial diversity and richness between the two soils at day 200, indicating that long-term straw amendment probably lessens the difference in bacterial community structure between the two soils. In the amended soils bacterial diversity, richness, and community composition at 25% of the water-holding capacity distinctly differed from those at 55% of the water-holding capacity, indicating that moisture strongly affects bacterial distribution in the amended soils. As stubble-C availability declined over time, the dominance of copiotrophic population weakened, and oligotrophic population was moderately abundant. Finally, our study suggests that dissolved organic carbon, which drives redistribution in copiotrophic and oligotrophic categories in response to the varying water and stubble-C availability, is a determinant of bacterial community composition in the amended soils.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Applied Soil Ecology - Volume 86, February 2015, Pages 62–70
نویسندگان
, , , , ,