Incorporation of decade old soil carbon into the soil animal food web of an arable system

Soil organic matter (SOM) is the main energy resource of the great diversity of soil invertebrates but knowledge on the contribution of SOM pools of different ages to soil animal nutrition is sparse. In the present study natural stable isotope ratios were used to investigate the incorporation of decade old wheat-borne carbon into soil invertebrates of different trophic levels of a maize field 27 years after the replacement of wheat. For comparison a nearby continuous wheat field was investigated. Although the soil food web of the wheat and the maize field consisted of similar invertebrate taxa, most taxa were significantly more abundant in the wheat than in the maize field confirming that C4 plants are generally of poor food quality to soil animals due to low nutrient and high fibre contents. However, different cultivation strategies may also have contributed to differences in the abundance of invertebrates between both study sites. The mean incorporation of C3 carbon in soil animals in the maize field was 60.6 ± 27.8%, but significantly differed between invertebrate species. Unfortunately, the field size did not entirely exclude incorporation of C3 carbon into the tissue of large and mobile invertebrates by feeding on C3 resources outside of the maize field. However, less mobile species such as endogeic earthworms incorporated high amounts of C3 carbon presumably due to the mobilisation of old carbon pools enclosed in soil aggregates and inaccessible to other soil invertebrates during gut passage. Furthermore, small and less mobile invertebrate species, such as most hemi- and epiedaphic Collembolans, also incorporated high amounts of C3 carbon likely by feeding on saprophytic fungi and microorganisms. In contrast, euedaphic Collembola incorporated only small amounts of C3 carbon suggesting preferentially feeding on maize resources. Overall, our data suggest that decade old carbon resources form an important component of the soil animal food web, but that the exploitation of old carbon resources by animal species varies with their distribution within the soil matrix, trophic position and exploitation of algae, and as an artefact to the small size of the study site, animal mobility.