کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4392298 1618160 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Brominated phenols as auxin-like molecules
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش خاک شناسی
پیش نمایش صفحه اول مقاله
Brominated phenols as auxin-like molecules
چکیده انگلیسی
2,6-Dibromophenol (DBP) was reported as an auxin-like molecule using molecular quantum similarity measures. In this study, the auxin activity of this molecule and its chlorinated homologue is further determined using a bacterial biosensor: the auxin-inducible ipdC promoter of Azospirillum brasilense. We were able to demonstrate that DBP can induce gene expression, but to a lesser extent than the auxin indole-3-acetic acid (IAA) and that DBP is not an antagonist for the IAA signalling pathway. To investigate the role of the bromine groups, the molecule 2,6-dichlorophenol (DCP) was also tested for gene expression induction. However, no induction could be observed. In a second part, DBP and other molecules were modelled in the auxin-binding pocket of the plant auxin receptor TIR1 to evaluate theoretical binding energies. Both DBP and its chlorinated homologue DCP are not strong ligands compared to other known auxins such as IAA. The importance of a carboxylated side chain for optimal binding (and probably auxin activity) was demonstrated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Soil Biology - Volume 45, Issue 1, January–February 2009, Pages 81-87
نویسندگان
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