کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4392300 1618160 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Azospirillum brasilense PII proteins GlnB and GlnZ do not form heterotrimers and GlnB shows a unique trimeric uridylylation pattern
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش خاک شناسی
پیش نمایش صفحه اول مقاله
Azospirillum brasilense PII proteins GlnB and GlnZ do not form heterotrimers and GlnB shows a unique trimeric uridylylation pattern
چکیده انگلیسی

In many organisms, nitrogen metabolism is co-ordinated by a class of highly conserved proteins from the PII family. In Gram-negative bacteria PII proteins are trimers that can be covalently modified by uridylylation according to the cellular nitrogen status. Several prokaryotes have more than one gene that code for PII proteins. In Escherichia coli it was shown that the two PII proteins (GlnB and GlnK) can form heterotrimers and it was suggested that heterotrimerization of PII proteins could be widespread in Bacteria. The nitrogen-fixing plant-associative bacteria Azospirillum brasilense code for two PII proteins, GlnB and GlnZ. The expression of glnB and glnZ genes are induced under nitrogen fixing conditions and these proteins control both the expression and the activity of the nitrogenase enzyme. Here we show that unlike E. coli PII proteins, A. brasilense GlnB and GlnZ, do not form heterotrimers in vitro. Our data also suggest that A. brasilense GlnB shows a unique uridylylation pattern.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Soil Biology - Volume 45, Issue 1, January–February 2009, Pages 94–99
نویسندگان
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