Characteristics of coelomocytes of the stubby earthworm, Allolobophora chlorotica (Sav.)

Coelomic fluid and cells (coelomocytes) were extruded by the electric shock (1 min, 4.5 V) from field-collected individuals of Allolobophora chlorotica (Sav.). Coelomocytes were subjected to bright field and fluorescence light as well as electron microscopy (transmission and scanning). Results of all methods combined, revealed two distinct populations of circulating cells in the coelomic fluid: (1) autofluorescent eleocytes/chloragocytes filled with numerous large granules (chloragosomes) containing fluorophores, mainly riboflavin; and (2) amoebocytes, which can be differentiated into: (a) hyaline amoebocytes, firmly spreading in form of crinoline-like membranes; (b) granular amoebocytes with numerous dark granules spreading in form of fine pseudopodia. Flow cytometry confirmed the presence of highly complex autofluorescent cells (eleocytes/chloragocytes) and two cohorts of not-autofluorescence much less complex amoebocytes.