کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4392474 1618171 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Metallothionein expression and Neutral Red uptake as biomarkers of metal exposure and effect in Eisenia fetida and Lumbricus terrestris exposed to Cd
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش خاک شناسی
پیش نمایش صفحه اول مقاله
Metallothionein expression and Neutral Red uptake as biomarkers of metal exposure and effect in Eisenia fetida and Lumbricus terrestris exposed to Cd
چکیده انگلیسی

In this study we compared short-term, sub-lethal responses of two different earthworm species, Eisenia fetida and Lumbricus terrestris, exposed to Cd (100 mg/kg) under laboratory conditions. Biological responses at the cellular and molecular genetic levels of organisation were measured. First, the Neutral Red uptake test was performed on extruded coelomocytes. Observations showed a significant reduction in cell membrane integrity in E. fetida exposed to Cd, whilst L. terrestris membranes appeared to be unaffected. Second, metallothionein 2 (MT2) gene expression levels were measured by Q-RT-PCR. Observations showed a significant up-regulation of the Cd-inducible metalloprotein in both species. Transcriptome measurements indicated that basal MT gene expression levels were higher in E. fetida than in L. terrestris, but Cd-induced MT2 up-regulation was approximately similar in samples from the two species. In conclusion, the susceptibility of coelomocyte membranes to Cd-evoked toxicosis is not correlated with Cd-induced MT2 expression levels.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Soil Biology - Volume 43, Supplement 1, November 2007, Pages S233–S238
نویسندگان
, , , , ,