Aquatic macrophyte diversity assessment: Validation of a new sampling method for circular-shaped lakes

We propose and validate a new sampling method to assess the presence, abundance and distribution of macrophytes in circular-shaped lakes according to the requirements of the Water Framework Directive (WFD2000/60/EC). The results of the macrophyte survey, and in particular of macrophyte diversity, obtained using this method are also discussed.The sampling is based on randomly selected transects homogeneously distributed around the perimeter of the lake. The number of transects is proportional to the lake's size. The method was validated on six Italian volcanic lakes using computational resampling procedures on a total of 126 transects.Using resampling procedures, we show that the proposed approach identifies more than 75% of the overall species richness through a moderate sampling effort. According to our results, Charophytes dominate aquatic vegetation in Italian volcanic lakes. Species diversity is highest at shallow depths, whereas the most abundant species, such as Chara polyacantha, are located at an intermediate depth between the shoreline and the maximum growing depth.