کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4503700 | 1624242 | 2016 | 10 صفحه PDF | دانلود رایگان |
• P. oligandrum inocula colonized the rhizosphere throughout the experimental period.
• Plant root system colonization by P. oligandrum reduced P. chlamydospora necroses.
• Plant molecular responses differed according to treatments.
• P. oligandrum enhanced certain gene expression in P. chlamydospora infected plants.
Biological control of Phaeomoniella chlamydospora, a pathogen involved in Esca, a grapevine trunk wood disease, was performed using the oomycete, Pythium oligandrum. Three 4-month greenhouse assays showed that necrosis of Vitis vinifera L. cv. Cabernet Sauvignon cuttings caused by P. chlamydospora was significantly reduced (40–50%) when P. oligandrum colonized the plant root systems. The expression of a set of 22 grapevine defense genes was then quantified by real-time polymerase chain reaction to determine plant responses in the interaction between P. oligandrum/V. vinifera L./P. chlamydospora. In the trunk, specific grapevine responses to the different treatments (control, P. oligandrum, P. chlamydospora and P. oligandrum + P. chlamydospora treatments) were significantly differentiated. Expression levels of 6 genes associated with P. chlamydospora infection showed higher induction than when plants were pre-treated with P. oligandrum. These genes are involved in various pathways (PR proteins, phenylpropanoid pathways, oxylipin and oxydo-reduction systems).
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Journal: Biological Control - Volume 92, January 2016, Pages 7–16